Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVHPMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVEAAQQKLKAATTREE--QMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARYTEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKT-MRTMLIVSPAMIVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPRKDVTESAKPADNKQPKNSTNNKGRNAGKQQKRK 3WO7 Chain:A ((8-240)) --------------------------------ESEGIWNHFFVYPMSWLITTVANLLNGSYGLSIIIVTILIRLALLPLTLKQQKSM----RAMQVIRPEMEAIQKKYKEKASKDPKVQQEMQKELLGLYQKHGVNPMAG--CLPLFIQLPILMAFYFAIMRTEEIRYHTFLWFDLGQPDYILPFVAGITTYFQ-FKMTMSH-------QMKVMLYVMPVMIIIAGLSLPSALSLYWVIGNIFMIIQTYFIVVKAPP-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3WO7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 689 -101264 -146.97 -486.84 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -146.97 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.289

(partial model without unconserved sides chains): PDB file : Tito_3WO7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WO7-query.scw PDB file : Tito_Scwrl_3WO7.pdb: