Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYTIMEIINYLFGGISMKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKYHTNQHFLPDLTIPESIIGYKEL--AEAVDNADAILFVVPTKAIRSVAQELIGKMNTKP-IIIHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQKAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMTRGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLENMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMENEFSLR 1Z82 Chain:A ((16-325)) -------------------RFFVLGAGSWGTVFAQMLHENGEEVILWARRKEIVDLINVSHTSPY------VEESKITVRATNDLEEIKKEDILVIAIPVQYIR----EHLLRLPVKPSMVLNLSKGIEIKTGKRVSEIVEEILG----CPYAVLSGPSHAEEVAKKLPTAVTLAGENSKE---LQKRISTEYFRVYTCEDVVGVEIAGALKNVIAIAAGILDGFGGWDNAKAALETRGIYEIARFGMFFGADQKTFMGLAGIGDLMVTCNSRYSRNRRFGELIARGFNPLKLLESSNQVVEGAFTVKAVMKIAKENKIDMPISEEVYRVVYEGKPPLQSMRDLMR------------

General information: TITO was launched using: RESULT: Template: 1Z82.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1840 -53300 -28.97 -173.61 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -28.97 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_1Z82.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Z82-query.scw PDB file : Tito_Scwrl_1Z82.pdb: