Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSIMVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEE-YNELPIIANVAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKVSSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGLSGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPYICPKLIKELPVRMSELGIESLERLIKEVREERER 5KSW Chain:A ((1-306)) ---NRLSVKLPGLDLKNPIIPASGCFGFGEEYAKYYDLNKLGSIMVKATTLHPRFGNPTPRVAETASGMLNADGLQNPGLEVIMAEKLPWLNENFPDLPIIANVAGSEEDDYVAVCAKIGDAPNVKVIELNISCPNVKHGGQAFGTDPDVAAALVKACKAVSKVPLYVKLSPNVTDIVPIAKAVEAAGADGLTMINTLMGVRFDLKTRKPVLANITGGLSGPAIKPVALKLIHQVAQVVDIPIIGMGGVESAQDVLEMYMAGASAVAVGTANFADPFVCPKIIEKLPEVMDQYGIDSLENLIQEVKNSK--

General information: TITO was launched using: RESULT: Template: 5KSW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1843 -86848 -47.12 -284.75 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.94 3D Compatibility (PKB) : -47.12 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.592

(partial model without unconserved sides chains): PDB file : Tito_5KSW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5KSW-query.scw PDB file : Tito_Scwrl_5KSW.pdb: