Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQP-ETPIFEASLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDGSQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKPLLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGGTTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKIAK 1L1T Chain:A ((2-274)) -PELPEVETIRRTLLPLIVGKTIEDVRIFWPNIIRHPRDSEAFAARMIGQTVRGLERRGKFLKFLLDRDALISHLRMEGRYAVASALEPLEPHTHVVFCFTDGSELRYRDVRKFGTMHVYAKEEADRRPPLAELGPEPLSPAFSPAVLAERAVKTKRSVKALLLDQTVVAGFGNIYVDESLFRAGILPGRPAASLSSKEIERLHEEMVATIGEAVMKGGS--------------FQHHLYVYGRQGNPCKRCGTPIEKTVVAGRGTHYCPRCQR-----

General information: TITO was launched using: RESULT: Template: 1L1T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1302 -29829 -22.91 -115.61 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -22.91 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.466

(partial model without unconserved sides chains): PDB file : Tito_1L1T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1L1T-query.scw PDB file : Tito_Scwrl_1L1T.pdb: