Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGIMGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLVFPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWLDQVIFSNNPLELVNELRAVSIKNQLLLKEAK 1T35 Chain:A ((4-179)) --ICVFAGSNPGGNEAYKRKAAELGVYMAEQGIGLVYGGSRVGLMGTIADAIMENGGTAIGVMPSGLFSGEVVHQNLTELIEVNGMHERKAKMSELADGFISMPGGFGTYEELFEVLCWAQIGIH-QKPIGLYNVNGYFEPMMKMVKYSIQEGFSNESHLKLIHSSSRPDELIEQMQNY------------

General information: TITO was launched using: RESULT: Template: 1T35.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 828 -114955 -138.83 -653.15 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -138.83 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.541

(partial model without unconserved sides chains): PDB file : Tito_1T35.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1T35-query.scw PDB file : Tito_Scwrl_1T35.pdb: