Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEFTNS----------DSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDINMGCPVPNVAERGKGSGLILRPEV---AAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISHLLHQDIANLSVHLRTRKEMSKVD-AHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGEELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSITGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ 3W9Z Chain:A ((3-242)) -------------VLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRIC-PELQNASR-----TPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGC-------------LLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQAGATELVVHGRTKEQGYRAEHIDWQAIGDI---RQRL--NIPVIANGEIWDWQSAQQCMAISGCDAVMIGRGALNIP----------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3W9Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1100 9999 9.09 49.50 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 9.09 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_3W9Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3W9Z-query.scw PDB file : Tito_Scwrl_3W9Z.pdb: