Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPASQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKHIDEEKILLAIRPEKDVDGFHPMNLGRLLAG-KPDKIPCTPYGIMKMFEAYSIDPAGKRAVVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEFVKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKSAEAFGAGE 3P2O Chain:A ((6-281)) ---LLDGKALSAKIKEELKEKNQFLKSKGIESCLAVILVGDNPASQTYVKSKAKACEECGIKSLVYHLNENITQNELLALINTLNHDDSVHGILVQLPLPDHICKDLILESIISSKDVDGFHPINVGYLNLGLESGFLPCTPLGVMKLLKAYEIDLEGKDAVIIGASNIVGRPMATMLLNAGATVSVCHIKTKDLSLYTRQADLIIVAAGCVNLLRSDMVKEGVIVVDVGINRLESGKIVGDVDFEEVSKKSSYITPVPGGVGPMTIAMLLENTVKSAK------

General information: TITO was launched using: RESULT: Template: 3P2O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1520 -118732 -78.11 -431.75 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -78.11 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.702

(partial model without unconserved sides chains): PDB file : Tito_3P2O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3P2O-query.scw PDB file : Tito_Scwrl_3P2O.pdb: