Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEGQAVSYDVEDGQRGPQATNIVKA 2LXJ Chain:A ((1-66)) MEQGTVKWFNAEKGFGFIERENGDDVFVHFSAIQGDGFKSLDEGQAVTFDVEEGQRGPQAANVQKA

General information: TITO was launched using: RESULT: Template: 2LXJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 275 -23060 -83.85 -349.39 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.96 3D Compatibility (PKB) : -83.85 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.96 QMean score : 0.604

(partial model without unconserved sides chains): PDB file : Tito_2LXJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LXJ-query.scw PDB file : Tito_Scwrl_2LXJ.pdb: