Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVETAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF-FSLISGSLL---FSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNYYVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE 3UON Chain:A ((27-85)) -------------------------------------------------------------------VLVAGSLSLVTIIGNILVMVSIKVNRHLQTVNNYFLFSLACADLIIGVFSMNLYTLYTV--------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3UON.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 108 -17835 -165.14 -324.27 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.46 3D Compatibility (PKB) : -165.14 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.46 QMean score : 0.229

(partial model without unconserved sides chains): PDB file : Tito_3UON.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UON-query.scw PDB file : Tito_Scwrl_3UON.pdb: