Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNC-QVSLTTVIRTLKEF------KSYLPKQSKRILP-RVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIKVR 5HPF Chain:A ((8-96)) ------------------------------------------------------------------------------------------------------------------------------KVAQSFLNLLCAQTSLTFSIVVLDEHEVVPVARSYLPQQDNRVSPYGMHLGNRLPAHATSTGKVLLSVLDREVQ--IEWIEKYGLKRLTPY------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5HPF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 253 1838 7.26 22.69 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : 7.26 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.106

(partial model without unconserved sides chains): PDB file : Tito_5HPF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HPF-query.scw PDB file : Tito_Scwrl_5HPF.pdb: