Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVLPPKINVPATPALDGVDINSLYDVNHGKLLGKGGFSEVLAVRHIPSGEIRALKVMMRSSLVGKKAEMVAHEKEILRRTCHPAIITLHEAVQTPDKVYFALDLM-NEDLFEFIVRNKTVNEDLSRAIMHQLMSGIAYLHEQSIVHRDIKPENILINVVVKSEANNAANDDSESATRVEGLQVMSDINSIPLEKLNVEVKIADFGLAKVVMEWDVCSTPCGTSFYIAPEVIRGIEEQGAKPLCTNQRLVKSVDVWSAGVVFYVLLCGRPPFHGQVRTGQDRRDLLRRIDHGVLFNPNHGWDSISAEAKNLILKMLDQESSKRITSDEVLRHPFFTAHGYSRPVPASDARRRFMQMQQLSLRTKVPAAQSAEAQVKTMSPLSGPDGQQIKVSSSGGGSSSKGSSNSTGSFLSSIKDFFGHRSKHTSDISKEERQRMHAELAELQATVIAEEDQEGDVTSYKPSMPVKEAKPARTAVMNMKAKVGPDALRK 5JZJ Chain:A ((28-294)) -------FQIPAT-------ITERYKV--GRTIGDGNFAVVKECVERSTAREYALKIIKKSKCRGKE-HMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTNKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLVY---------EHQDGSKS------------------------LKLGDFGLATIVD--GPLYTVCGTPTYVAPEIIA---ETGYG---------LKVDIWAAGVITYILLCGFPPFRG---S----EVLFDQILMGQVDFPSPYWDNVSDSAKELITMMLLVDVDQRFSAVQVLEHPW------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5JZJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1315 -85511 -65.03 -326.38 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -65.03 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.462

(partial model without unconserved sides chains): PDB file : Tito_5JZJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JZJ-query.scw PDB file : Tito_Scwrl_5JZJ.pdb: