Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALVKTKLAAAPQPSRTPTPGSSPKEWTPPTHTHESPEHLSTDEPDVLVPAHKYVYDVKTCAWKGVDTMLRVLHPNRGLSQGTMRVCFALEELDETGFSSRMVAKMFRHNISKVVESD-YFNEGEAQCICGIFAEKFNRVQVPAGFQRHIVSFLQCETVRIKLSEVPEAYQHKRSGFFSYRTTDSADILFTMEPRLAGNFTKYTNNFGDVYDGFERRQSLEEEKKRHRVLMAVEAFSHFTLVESGGSMLVCDLQGVNDFLTDPQIHTEDGKGLGMGNMGQDGIRKWMEAHVCNEVCRAIMLEPLSKGLRNFTRTAENESRVSYYQILRAKLRSQTPVRPEDIIPLSKPLSLMSDDERLEYAMRLSALLSE 4ZMF Chain:A ((97-272)) ------------------------------------------------------------------------------------------EAMTQLKNGTKFVLKLYKKEA----SRELYFEDVKMQMVCRDWGNKFNQKKPPKK-----IEFLMSWVVEL-IDRSP--------------SSNGQPILCSIEPLLVGEFKKNNSNYGAVLTNRSTPQ----------------AFSHFTYELSNKQMIVVDIQGVDDLYT-PQIHTPDGKGFGLGNLGKAGINKFITTHKCNAVCALLDLD--------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4ZMF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 770 -3097 -4.02 -18.21 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -4.02 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.418

(partial model without unconserved sides chains): PDB file : Tito_4ZMF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZMF-query.scw PDB file : Tito_Scwrl_4ZMF.pdb: