Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKEKMFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGENIVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNV--ALSRFLNLDSVDIVTGFNIPLLVELISSYDSKINLEEIVHNAQNSLFNVKQQLNVEEEEDLCL 4TKZ Chain:A ((4-102)) ----------IIIVAHGNFPDGILSSLELIAGH--QEYVVGINFIAGMSSNDVRVALQREVIDF----KEILVLTDLLGGTPFNVSSALSVEYTDKKIKVLSGLNLSMLMEAVLS-----------------------------------

General information: TITO was launched using: RESULT: Template: 4TKZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 427 -68892 -161.34 -710.22 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -161.34 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_4TKZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TKZ-query.scw PDB file : Tito_Scwrl_4TKZ.pdb: