Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSAIKPRSTKKLSQIIVEQLIEKIQSGEFKVGEKIPTELELIETFEVSRSVIREAITELRSLGFVETRHGIGTFVKE--QTVEQNFLLSNASL--ETINDII-SLLELRISLESEAVFLASERRKQIHIDKMKAALEDFERHISSDANDGTVKADYDFHIAIAEASENQYFVDFLKYLGEKIIPRARVKSIEQSPENREEYLKAVHHDHVNIYNAIVDQDGLLARQMMRAHLSKSIKKFKQ 1H9G Chain:A ((18-165)) ------------------EYIIESIWNNRFPPGTILPAERELSELIGVTRTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLLSVRTNIST--IFIRTAFRQ--HPDKAQEVLATANE--VADHADAFAELDYNIFRGLAFASGNPIY----------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1H9G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 394 -23548 -59.77 -164.67 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -59.77 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_1H9G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1H9G-query.scw PDB file : Tito_Scwrl_1H9G.pdb: