Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPNTIFLQRVADLYDTFKQHDSQQSD-RLQRYRNIEVESAQLISQLIRMQQAKSILEIGTSTGYSTLWLAEAAQATGGQVITVEIDAKRSAEAKRHVAELELSEIVQFWVGDAADYLKAAQE--KFDFILLDAERDAYENYWPDLKRLMKPKGGVLVVDN---------------VISHA-AEVNRFIALIKKDPDYMSSILPVGAGLCLVVTK 4OA8 Chain:A ((29-220)) -----------DTEGALKAHKTAPSELRMAQLGTVE---GQMLQLLIRMAGIHSIVEVGTCVGFSAICMAHALPSK-GHIYTIEKDYENVVTANQNIVNCKLEDKITVLHGEALAQLNTLKEMAPFDMIFIDANKSSYLAYLNWAKMYIR-KGGLIVADNTFLFGSVFDEHPTEKVSSNAHASMRAFNDELANKEKYLSTIIPTSEGM------

General information: TITO was launched using: RESULT: Template: 4OA8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 861 -5677 -6.59 -32.82 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -6.59 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_4OA8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OA8-query.scw PDB file : Tito_Scwrl_4OA8.pdb: