Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLSLKSLQCFVTLVKTKSFTRTAEELYLTQPTISKILQQLEEQLQVQLLVKPDHGRKRQIELTEIGERIYQHAGELLQAEQNIFLEIENYQQLKTGTLKLGVPP-LGSQLLTTALFDFHRQWPDIELAFMEVGSRGIEQALLNNELDVGVLLQPFDEQTFNSIELCNYPLMVLLRRDATWATRKKINLEELQHQSFLMFPENFSLNSIILDACKQHGFYPTIACRTSQWHLLADMVLQRMGIALLPQYYTDMLDPTLFAAVPLEKPNIQWHLVMAWKKNLPVSPAVQAWLSIVRQHFQHIKP 3HFU Chain:A ((30-222)) -----------------------------------------------------------------------------------------------GSLRIAVTPTFTSYFIGPLMADFYARYPSITLQLQEMSQEKIEDMLCRDELDVGIAFAPVHSPELEAIPLLTESLALVVAQHHPLAVHEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVVIEANSISAVLELIRRTSLSTLLPAAIATQHDG--LKAISLAPPLLERTAVLLRRKNSWQTAAAKAFL------------

General information: TITO was launched using: RESULT: Template: 3HFU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 974 -40607 -41.69 -211.49 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -41.69 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.532

(partial model without unconserved sides chains): PDB file : Tito_3HFU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HFU-query.scw PDB file : Tito_Scwrl_3HFU.pdb: