Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFTKKDLLQQRMLIDQLRTQNSLSPQNAAKLGQSIASSWERSAS-AAIPKERFAAPLL--EK--KSASQNALDLALSQCADDLRHIAEQSSMVIAVGDIGSTIIWTASSAQMQSAAERVHFVQGGQWREEFVGTNALALSLKTQQSSCVFSNEHYMESIHDWVCYAAPIIDPYSKQTLGVVDLSTTWKNHNTLGILAAERCASIIQSALLEQQRQQLHIRAFSTPQVKFNGKSLLLTPRQIEILTILALCPHGLTLENLYQALYGERKVSMGTLKAEMSQLRDILGGLLGSRPYRLLVHVEADFLQAEQALDAGYVASALQLYAGVFLAKTESPFLCAWRDCLESRLSDAIFKAQETDLLLKHLAHFPEAIDAVERLMELLPSEHPAHQLLVKYIDSPKLS 4LRX Chain:D ((12-166)) ------------------------------ISPLIATSWERCNKLMKRETWNVPHQAQGVTFASIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQVLWNWAFCATPLFDS-KGRLTGTIALACP-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4LRX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 674 -957 -1.42 -6.38 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain D : 0.57 3D Compatibility (PKB) : -1.42 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.402

(partial model without unconserved sides chains): PDB file : Tito_4LRX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LRX-query.scw PDB file : Tito_Scwrl_4LRX.pdb: