Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIVERPSYTSKDVEVTSREPLFSGFIQVEKVSLRHRLFNQSEYTPVLQRELVHRPEAAGVLLYNDQKQQFALIEQFRVGALDDSHSPWQL-EIIAGVLDGNESPESCIRCESLEESGCEVQDLEHLFSFYPSAGACSELFHLYVAETN-LPAVGGVFGVDNEGENIQLHLFSYSEIQTLLNSGRLRNAPVIMALQWLAQHSKTIINPKR 3O69 Chain:A ((38-184)) ------------------------------------------------KREVYDRGNGATILLYNTKKKTVVLIRQFRVATWVNGNESGQLIESCAGLL--NDEPEVCIRKAAIEETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSD--------------EDIEVLELPFSQALEMIKTGEIRDGKTVLLLNYL------------

General information: TITO was launched using: RESULT: Template: 3O69.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 600 -28016 -46.69 -212.24 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -46.69 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.457

(partial model without unconserved sides chains): PDB file : Tito_3O69.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3O69-query.scw PDB file : Tito_Scwrl_3O69.pdb: