Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMDYQNAVQAFDLESTAKQMLSGSLDALNACYECCDRH--ADGDKIALYWQGKDGRKEQY-TFRELKEWSSQFANFLKSQGVKAGDRISGLLPRTPELIVTILAAWRIGAVYQPLFTAFGPKAIEHRIQLAQSKLVVT-DMGNRS--------KLDEIEKCPAIMTVADAQGTPLKAGDFNF-------WNE-VKQQSDQCDLVMRSIQDPFLLMFTSGTTG-PAKPLEVPLKALIAFGRYMQDAIGLTEEDSFWNIADPGWAYGLYYAITGPLFLGHATLFYEGGFSIDS---LCQIVKDYKVNNLAGAPTAYRMMMA-ADPAQMAPLKGQFRVVSSAGEPLNPEVIRWFKQVL---DAPIYDHYGQTEVGMVVCNHHGLKHEIHAGSAGFPSPGYRVAIVNEQGEELPPDTPGILAVDIS---QSPMMWFGGYKESRKSPF--IGHYYLTGDTAELHADGSMSFVGRSDDVITTSGYRIGPFDVESALLEHDAVIEAAVIGVPDPDRTEVVKAFVILAAGIQPSDALAEELSQFVKRRLSAHAYPRLVEFVSELPKTPSGKIQRFLLRNQEIAKQQAKAG 2P2M Chain:A ((75-621)) ---------------------------LNLAANCLDRHLQENGDRTAIIWEGDDTSQSKHISYRELHRDVCRFANTLLDLGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPEAVAGRIIDSSSRLVITADEGVRAGASIPLKKNVDDALKNPNVTSVEHVIVLKRTGSDIDWQEGRDLWWRDLIEKASPEHQPEAMNAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAATTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVPNWPTPARMCQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINPEAWEWYWKKIGKEKCPVVDTWWQTETGGFMITPLPGAIELKAGSATRPFFGVQPALVDNEGHPQEGATEGNLVITDSWPGQARTL-FGDHERFEQTYFSTFKNMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHAIKGQAIYAYVTLNHGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILR------------

General information: TITO was launched using: RESULT: Template: 2P2M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3083 -37837 -12.27 -74.34 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -12.27 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_2P2M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P2M-query.scw PDB file : Tito_Scwrl_2P2M.pdb: