Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDILENPLELCGFAFIEFVSKENELDPIFETIGFSKVAKHKSKKAYLWRQGNINIILNYQPESYASFFFNEHGPSACAMGFKTRDAAKAFKKAVELGAEPMYSKVGPMELN----IPAIKGIGGMPIFLVDC-DIYENDFV----FFDDAQRNPEGAGLKEIDHLTHNV--YKGRMEYWANFYEKIFNFQEIRYFDIKGEYTGLTSKALTAPDGMIRIPLNE--DSDKGNGQIAEFLADFNGEGIQHIAFICDDLISTWDKLKGL----GLKFMTPPPNTYYEMLEERLPE--HGEPTEELKQRCILLDGSTKDGQKKLLLQIFSENM--IGPVFFEFIQR-----KDDDGFG----------EGNFKALFESIERDQIRRGVLEAK 1SP9 Chain:A ((131-428)) -------------------------------------------------------------------FFSSHGLGVRAVAIEVEDAESAFSISVANGAIPSSP---PIVLNEAVTIAEVKLYGDVVLRYVSYK------FLPGFERVE-----PLDYGIRRLDHAVGNVPELGPALTYVAGF-TGFHQFA-----------SGLNSAVLASNDEMVLLPINEPVHGTKRKSQIQTYLEHNEGAGLQHLALMSEDIFRTLREMRKRSSIGGFDFMPSPPPTYYQNLKKRVGDVLSDDQIKECEELGILVD---RDDQGTLL-QIFTKPLGDRPTIFIEIIQRVGCMMKDEEGKAYQSGGCGGFGKGNFSELF----------------

General information: TITO was launched using: RESULT: Template: 1SP9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1060 -8815 -8.32 -37.04 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -8.32 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_1SP9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SP9-query.scw PDB file : Tito_Scwrl_1SP9.pdb: