Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMNSSTEQIISNSLSLRLKQETAAEHERMHQLMSE--AKVFSSKEKYAQFTLSQYYFQLEIEHLFEKEGV--AGL---IP--DLDIRGRSKQALADLNDLGIQPNGQ------Q---------LQSENVQLPEALGWIYVSEGSTL-GAAFLFKEAQKHLGFSETFAARNLAAYPEGRAKVWKRFVKALDEAG--FDQTQQDRVVQGALDAFGYFGQALDQLDELK 4RAJ Chain:A ((18-230)) -----------EPLTKRLSKASRKIHNVSNSLVNARLIALFSDKDLYAKALGCFYYVFVALEAALDEALKKGDADVSKFKDVLKGGLYRAPGFKQDVQHYLGATWQAQLGTKSQALKDYEAHLASLGRSSPALLLAHVYTQHLAMASGGQIVKRWARKIFQLPDDVGTAAFDYTGESNNTLRSAFKKQFDEWGAAQPQELQDQLLSEHLAAFGHNNAIIKAFPL--

General information: TITO was launched using: RESULT: Template: 4RAJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 696 17818 25.60 95.79 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 25.60 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_4RAJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RAJ-query.scw PDB file : Tito_Scwrl_4RAJ.pdb: