Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFYKHTSLLAERITAHGALPNTSYWRGFFFSGVCMTEFQKITHEIRQLQVELNHLGSCNTKGLTTEQIAHLDERFFLAIAKQNKLLARLNNKPEGFL 3KWO Chain:A ((52-72)) ---ELFDSCAERVLQLGEKAITCQ-------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3KWO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 16 -1591 -99.44 -75.76 target 2D structure prediction score : 0.95 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -99.44 2D Compatibility (Sec. Struct. Predict.) : 0.95 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.815

(partial model without unconserved sides chains): PDB file : Tito_3KWO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3KWO-query.scw PDB file : Tito_Scwrl_3KWO.pdb: