Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQTLIFMHETGKISIDFFKECIMQDIPMSRWLSPLMAFCLSFVIMATLAPTMGIQIDRQIDFWLLWFGTMLLLALPVCYLEIALAKRSKTTALNALSSLTREADSSPKWRVVGCLAVVFIPFLA------------------GNVLST-------------------------ASNILVAQ----F------APS-ISSQ-----IIFVGLAVAALVLSFIP--R--QILILLMTLGVIASIVLANMM-GS-----TLQPWHWTSV-EFKEWGNATVLALVASGLGLGLYW-QNSVGAVQAQEGATKTVLPIWLAQLIAVVAFGFFSL--------------------------------QAQLPVLTWI---------FTGVMTSALFVQLAREQLAQRQLMPVLQ--WVIIVVAIAVWAVPEVHNL------FTLIL-MQ--WGL-LICLIYAVFAGWIMKISHLRKSMNFSN-ELFYNLWRIAVRIVLPLSIIVAMIAVIGQSI 4XP9 Chain:C ((27-474)) ----------------------------------------------WRFPYLCYK-NGGGAFLVPYGIMLAVGGIPLFYMELALGQHNRKGAITCWGRL------VPLFKGIGYAVVLIAFYVDFYYNVIIAWSLRFFFASFTNSLPWTSCNNIWNTPNCRPFEGHVEGFQSAASEYFNRYILELNRSEGIHDLGAIKWDMALCLLIVYLICYFSLWKGISTSGKVVWFTALFPYAVLLILLIRGLTLPGSFLGIQYYLTPNFSAIYKAEVWVDAATQVFFSLGPGFGVLLAYASYN--KYHNNVYKDALLTSFINSATSFIAGFVIFSVLGYMAHTLGVRIEDVATEGPGLVFVVYPAAIATMPASTFWALIFFMMLATLGLDSSFGGSEAIITALSDEFPKIKRNRELFVAGLFSLYFVVGLASCTQGGFYFFHLLDRYAAGYSILVAVFFEAIAVSWIYGTNRFSEDIRDMIGFPPGRYWQVCWRFVAPIFLLFITVYGL----

General information: TITO was launched using: RESULT: Template: 4XP9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1376 -197710 -143.68 -612.11 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain C : 0.61 3D Compatibility (PKB) : -143.68 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.221

(partial model without unconserved sides chains): PDB file : Tito_4XP9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XP9-query.scw PDB file : Tito_Scwrl_4XP9.pdb: