Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDLYYWGTPNGHKITIALEEMGLDYTLYPINILENDQFQPDFLKISPNNKIPAIVDQDGPRGEPISVFESGAILQYLGRKTGLFYPTDEQERVEVEQWLMWQMGGLGPMLGQNHHFNRFA----PEKIPYAIDRYVNETKRLYGVLNKQLI-RQKFVAGEYSIADMAILPWILRY-----EWQGIQLEDYPYVQEYIVRLTARPAVQKALSIKVI 4NHZ Chain:A ((43-252)) -IQLYSLPTPNGVKVSIMLEETGLPYEPHAIDFGKDHQKTPEFLSLNPNGKIPAIIDPNGPGDKPLGLFESGAILQYLAEKTGQFLPADPARRWQTLQWLHFQMGGIGPMFGQLGFFHKFAGREYEDKRP--LQRYVAESKRLLGVLEARLDGRQWIMDADYTIADIATLGWVRNLIGFYGARELVAFDELTHVPAWLERGLARPAVQRGLEI---

General information: TITO was launched using: RESULT: Template: 4NHZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 917 -67412 -73.51 -337.06 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -73.51 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_4NHZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NHZ-query.scw PDB file : Tito_Scwrl_4NHZ.pdb: