Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQDAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADFTEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMALSVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQSAPI-TKRNLNRKVTFSLI-SYSLLLFSGFLLLMVLLVIHT-SW-DITVWYHILNTFLFLLPIISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFVQNNNLISETLS-YNQT-------------------FAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN 5DO7 Chain:D ((472-671)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KCYSERAMLYYELEDGLYTTGPYFFAKILGELPEHCAYI--IIYGMPT-YWLANLRPGLQPFLLHFLLVWLVVFCCRIMALAAAALLPTFHMASFFSNALYNSFYLAGGFMINLSSLWTVPAWISKVSFLRWCFEGLMKIQFSRRTYKMPLGNLTIAVSGDKILSVMELDSYPLYAIYLIVIGLSGGFMVLYYVSLRFIKQKP-------

General information: TITO was launched using: RESULT: Template: 5DO7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 531 -63047 -118.73 -358.22 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain D : 0.55 3D Compatibility (PKB) : -118.73 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.311

(partial model without unconserved sides chains): PDB file : Tito_5DO7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DO7-query.scw PDB file : Tito_Scwrl_5DO7.pdb: