Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSILEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLRQQI--IGSDPEEELSGSALEE-QERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYYLVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV 5I8I Chain:A ((224-431)) -----------------------------------------------------------IAFKVMNKPDLLEDEYSREFPKNPISQY------PKDLTIAIPKEVPWFGETENPKLYTKAVASLKNTGAKIVVVDFEPLLELARCL-YEGAWV-----AERYCATRDFLATNPPESSLDETVVNIIKGAVKFDAADAFKFEY------------------

General information: TITO was launched using: RESULT: Template: 5I8I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 203 1228 6.05 9.59 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 6.05 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.257

(partial model without unconserved sides chains): PDB file : Tito_5I8I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I8I-query.scw PDB file : Tito_Scwrl_5I8I.pdb: