Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRFSYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSKTYTSGDKK-LYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNISQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGLESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLMDQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYEPGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSSNVGMVILEQRLGG----RWYNYLQKLGFGQSTHSGLDDEVNGALPTSNIVDRAMSAYGQAVGVTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEE-RTTQTEVLGQPFSKETTEKVREYMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIAS-DTGGYQTGDTAYLYSIVEMVPSEDPDYVLY----LTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYRNLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVTGWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE 5TRO Chain:A ((26-555)) -----------------------------------------------------------------------QQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRHVVDKK-----ETAKKLSTVINMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIE----KMNLPGISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSG--------------------------KRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAKTGEILAYSQRPTFNPETGKDFGKK---WANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDKKYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWY---ERFGFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKPWFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAAN--YRIDGYEVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAYELGVSKAFKPIMENTLKYLNVGKS--------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5TRO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2963 202843 68.46 410.61 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 68.46 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.477

(partial model without unconserved sides chains): PDB file : Tito_5TRO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TRO-query.scw PDB file : Tito_Scwrl_5TRO.pdb: