Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFRQALQLAACGLAGGSAAVLFSAVAVGKPRAGGDAEPRPAEPPAWAGGARPGPGVWDPNWDRREPLSLINVRKRNVESGEEELASKLDHYKAKATRHIFLIRHSQ-YHVD--GS------L-EKDRTLTPLGREQAELTGLRLASLGLKFNKIVHSSMTRAIETTDIISRHLPGVCKVSTDLLREGA--PIEPDP-------------PV--SHW------KPEAVQYYEDGARIEAAFRNYIHRADARQEEDSYEIFICHANVIRYIVCRALQFPPEG------WLRLSLNNGSITHLVIRPN----GRVALRTLGDTGFMPPDKITRS 2YN2 Chain:A ((11-246)) -----------------------------------------------------------------------------------------------AIENIYIARHGYRSNWLPKGPYPPPPTGIDNDVPLSEHGVEQAHELANYISKLDVKPEMIFSSPFYRCLETSKPTVEALK-IPLYVDRGVGEWYKPDRPIIPEPATHEVMSKFFPSMISPDWEPSIIPSNKGETEEDIFERCHKFWPVFIDRVERKFPNVKTIMIVTHAATKSALGMNLLKFSSAKEPIDNKGTFIRNGSCAIDKFEL---PFEEREWKLTMNGNTSFLTNG-----

General information: TITO was launched using: RESULT: Template: 2YN2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 957 18862 19.71 101.95 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 19.71 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_2YN2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YN2-query.scw PDB file : Tito_Scwrl_2YN2.pdb: