Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKDDQKYLLAGLYSLLVAIFYFPLIDSKGIFVSILMAVLLLYLIYFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQEYCQGQPYGLLKKMLKRIRLSREIALLLATIIVYFFTHRILPLSVFTFMFSYILLFVQSYLGSNTAWIGNRRLIIDDEFEKILLSKSYVKEISSARYSEYLTCEYKNLTPIILIAIFENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYDNEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIVVINEFIACLNSRCVPSQYDRFFYKDRPYIFSRKSPIKG 2YEW Chain:K ((40-104)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PSLVQDYITCNYKTVVPSPYIKCCGGAECSK---NEADYKCSVFTGVYPFMWGGAY-----------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2YEW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain K - contact count / total energy / energy per contact / energy per residue : 53 -5361 -101.14 -101.14 target 2D structure prediction score : 0.21 Monomeric hydrophicity matching model chain K : 0.44 3D Compatibility (PKB) : -101.14 2D Compatibility (Sec. Struct. Predict.) : 0.21 1D Compatibility (Hydrophobicity) : 0.44 QMean score : 0.151

(partial model without unconserved sides chains): PDB file : Tito_2YEW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YEW-query.scw PDB file : Tito_Scwrl_2YEW.pdb: