Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILITGANGQLGTELRYLLDERNEEYVAVDVA-------EMDITDAEMVEKVFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVAKASEKHGATLVYISTDYVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGK----NFVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENR----KEFGYYHLSNDATEDTTWYDFAVEILKDTDV---EVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQEFYKQEVR 4NDN Chain:E ((20-294)) -VLVTGATGLLGRAVHKEFQQNNWHAVGCGFRRARPKFEQVNLLDSNAVHHIIHDFQPHVIVHCAAERRPDVVENQ-PDAASQLNVDASGNLAKEAAAVGAFLIYISSDYVFDGTNPPYRE---EDIPAPLNLYGKTKLDGEKAVLENNLGAAVLRIPILYGEVEKLEESAVTVMFDKVQFSNKSANMDHWQQRFPTHVKDVATVCRQLAEKRMLDPSIKGTFHWSGN--EQMTKYEMACAIADAFNLPSSHLRPITDSPV-LGAQRPRNAQLDCSKLETLGI------------------

General information: TITO was launched using: RESULT: Template: 4NDN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1321 -364 -0.28 -1.41 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain E : 0.74 3D Compatibility (PKB) : -0.28 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.469

(partial model without unconserved sides chains): PDB file : Tito_4NDN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NDN-query.scw PDB file : Tito_Scwrl_4NDN.pdb: