Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTNRKDEHILYA----LEQKSSYNSFDEVELIHSSLPLYNLDEIDLSTEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAETCGILFVTGSYSAAL-KNPTDDSFS-VKSSHPNLLLGTNIGLDKPVE-LGLQTVEEMNPVL----LQVHVNVMQELLMPEGERKFRSWQ-SHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRRSGQR---------DYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRTVLELVETYTVEEVIGIVQGWKA---DLRLIMCSLNCATIADLQKVDYLLYGKLKEANDQMKKA 3B03 Chain:A ((5-349)) --VNRKVEHVEIAAFENVDGLSSSTFLNDVILVHQGFPGISFSEINTKTKFFRKEISVPVMVTGMTGGRNELGRINKIIAEVAEKFGIPMGVGSQRVAIEKAEARESFAIVRKVAPTIPIIANLGMPQLVKGYGLKEFQDAIQMIEADAIAVHLNPAQEVFQPEGEPEYQIYALEKLRDISKELSVPIIVKESGNGISMETAKLLYSYGIKNFDTSGQGGTNWIAIEMIRDIRRGNWKAESAKNFLD-WGVPTAASIMEVRYSVPDSFLVGSGGIRSGLDAAKAIALGADIAGMALPVLK----SAIEGKESLEQFFRKIIFELKAAMMLTGSKDVDALKKTSIVILGKLKE--------

General information: TITO was launched using: RESULT: Template: 3B03.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1929 32342 16.77 100.75 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 16.77 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.472

(partial model without unconserved sides chains): PDB file : Tito_3B03.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3B03-query.scw PDB file : Tito_Scwrl_3B03.pdb: