Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIDILTLFPEMFSPL-EHSIVGKAREKGLLDIQYHNFRE-NAEKARHVDDEPYGGGQGMLLRVQPIFDSFDAIEKKNPR---VILLDPAGKQFDQAYAEDLAQEEELIFICGHYEGYDER-IKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSHQDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLLEHYQLTVEEEKMLAEIKENKE 4YQ2 Chain:A ((9-249)) MWIGVISLFPEMFKAITEFGVTGRAVKHNLLKVECWNPRDFTFDKHKTVDDRPYGGGPGMLMMVQPLRDAIHTAKAAAGEGAKVIYLSPQGRKLDQGGVTELAQNQKLILVCGRYEGIDERLIQTEIDEEWSIGDYVLTGGELPAMTLIDAVARFIPGVL------------DGLLDCPHYTRPEVLEGLTVPPVLMSGHHEEIRKWRLKQSLQRTWLRRPELLEGLALTDEQRKLLKEAQ----

General information: TITO was launched using: RESULT: Template: 4YQ2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 937 35280 37.65 158.20 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : 37.65 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_4YQ2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YQ2-query.scw PDB file : Tito_Scwrl_4YQ2.pdb: