Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVVVDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKVLGCEGVIVTNAAGGIG--FGPGTLMAISDHINM---TGQNPLMGENLDDFGPRFPDMSRAYTPEYRATAHEVAKKL--NIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVAAHSGLKVLGISCITNFAAGFQEEL---NHEEVVEVTERVKGDFKGLLKAILAEL 1PF7 Chain:E ((13-282)) ---------TAEWLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYSEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLWKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTWKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANHEEVLAAGKQAAQKLEQFVSILMASI

General information: TITO was launched using: RESULT: Template: 1PF7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1471 -87464 -59.46 -336.40 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain E : 0.83 3D Compatibility (PKB) : -59.46 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.573

(partial model without unconserved sides chains): PDB file : Tito_1PF7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PF7-query.scw PDB file : Tito_Scwrl_1PF7.pdb: