Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHISESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEACKAVVRANEEYVPLYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGGLV-PTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKIEEVGSANFF--GITADNE---FVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNL------DRFVEAGACGTAAVISPIGGIQH-GDDFHVFYSETEVGP-VTRKLYNELTGIQFGDIEAPEGWIVKVD 2COJ Chain:A ((68-378)) -----------------------------WEKPHIKPLQNLSLHPGSSALHYAVELFEGLKAFRGVDNKIRLFQPNLNMDRMYRSAVRATLPVFDKEELLECIQQLVKLDQEWVP-YSTSASLYIRPTFIGTEPSLGVKKPTKALLFVLLSPVGPYF----FNPVSLWANPKYVRAWKGGTGDCKMGGNYGSSLFAQCEAVDNGCQQVLWLYGEDH-QITEVGTMNLFLYWINEDGEEELATPPLDGIILPGVTRRCILDLAHQWGEFKVSERYLTMDDLTTALEGNRVREMFGSGTACVVCPVSDILYKGETIHI--PTMENGPKLASRILSKLTDIQYGREE----------

General information: TITO was launched using: RESULT: Template: 2COJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1476 -70214 -47.57 -239.64 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -47.57 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.343

(partial model without unconserved sides chains): PDB file : Tito_2COJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2COJ-query.scw PDB file : Tito_Scwrl_2COJ.pdb: