Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTLFSKIKEVTELAAVSGHE-APVRAYLREKLTPHVDEVVTDGLGGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFKLLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTIVPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLRGAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKI----GDGTLIRFYDPGHLLLPGMKDFLLTTAEEAGIKYQYYCG--KGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQAFLQALVKKLDRSTVDLIKHY 4X8I Chain:A ((7-339)) -------MKKIIESPGVSGYEHLGIRDLVVDILKDVADEVKIDKLGNV--IAHFKG-SAPKVMVAAHMDKIGLMVNHIDKDGYLRVVPIGGVLPETLIAQKIRFFTEKGERYGVV-GVLPPHLR------I--DWDSIIVDVGASSREEAEEMGFRIG-TIGEFAPNFTRLSEHRFATPYLDDRICLYAMIEAARQLGEHE--ADIYIVASVQEEIGLRGARVASFAIDPEVGIAMDVTFAKQ-PNDKGKIVPELGKGPVM---DVGPNINPKLRQFADEVAKKYEIPLQVEPSPRPTGTDANVMQINREGVATAVLSIPIRYMHSQVELADARDVDNTIKLAKALLEEL-----------

General information: TITO was launched using: RESULT: Template: 4X8I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1883 -20851 -11.07 -65.77 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -11.07 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.473

(partial model without unconserved sides chains): PDB file : Tito_4X8I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4X8I-query.scw PDB file : Tito_Scwrl_4X8I.pdb: