Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSYQAVYQILSK-ETDYISGEKIAEKLSLSRTSIWKAIKRLEQEGIEINSIKNRGYKLMN-GDLILPEILEENLPIKVSFK-----PETKSTQLDAKEAIDLGHEANTLYLASYQTAGRGRFQRSFYSPQG-GIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNLTLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFT--IKDFPQELKEKAASL-FKATAPITR----NELIIEI---WRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYKGLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK 3V8J Chain:A ((2-322)) -KYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLKLEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL-VGNQSSFFILSDEQ---------------GQGLWMSVVLRPNVAFSMISKFNLFIALGIRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTE-MVANNDG-IEAIICGIGINLTQQLENFDESIRHRATSIQLHDKNKLDRYQFLERLLQEIEKRYNQFLTLPFSEIREEYIAASNIWNRTLLFTEN-KQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF----

General information: TITO was launched using: RESULT: Template: 3V8J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1394 47271 33.91 164.71 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 33.91 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.598

(partial model without unconserved sides chains): PDB file : Tito_3V8J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3V8J-query.scw PDB file : Tito_Scwrl_3V8J.pdb: