Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFERGNCTMIYSKEIVREWLDEVAERAKDHPEWVDVFEHCYTDTLDNTVEILEDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYANSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETFVAATKEILHLWTVEQ--DHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSDDCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYTTNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSIDDEVYQATRRTILSSENPYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMHESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR 2P0V Chain:A ((76-470)) ------------------------------------FENCFPNTLDTTVHFDGKEDTFVYTGDIHAMWLRDSGAQVWPYVQLANKDPELKKMLAGVINRQFKCINIDPYANAFNMNSE-GGEWMSDLTDMKPELHERKWEIDSLCYPIRLAYHYWKTTGDASVFSDEWLQAIANVLKTFKEQQRKDDAKGPYRFQRKTERALDTMTNDGWGNPVKPVGLIASAFRPSDDATTFQFLVPSNFFAVTSLRKAAEILNTVNR--KPALAKECTALADEVEKALKKYAVCNHPKYGKIYAFEVDGFGNQLLMDDANVPSLIALPYLGDVKVTDPIYQNTRKFVWSEDNPYFFKGSAGEGIGGPHIGYDMIWPMSIMMKAFTSQNDAEIKTCIKMLMDTDAGTGFMHESFNKNDPKNFTRAWFAWQNTLFGELILKLVN--

General information: TITO was launched using: RESULT: Template: 2P0V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2363 -42219 -17.87 -107.43 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -17.87 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_2P0V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P0V-query.scw PDB file : Tito_Scwrl_2P0V.pdb: