Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTANIMTTLKNQITDALKTSMRAKDMATVTVLRSL---------QAAIKQIEVDERIELDDAQVLAVIEKQIKQR---KESIKAFEGAGRDDLASKEQAEAEVLSQFLPEAMTEEELDSLIEQTIAAQEATSMKDMGKVMNSLRPIIAGRADPAQVSAKIKAKLA 1OSA Chain:A ((34-125)) -----------------------------TVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLSLMARKMKEQDSEEELIEAFKVFDRDGNGLISAAELRHVMTNLGEKLTDDEVDEMI--------------------------------------------

General information: TITO was launched using: RESULT: Template: 1OSA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 95 6063 63.82 75.78 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 63.82 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.552

(partial model without unconserved sides chains): PDB file : Tito_1OSA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1OSA-query.scw PDB file : Tito_Scwrl_1OSA.pdb: