Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKILITGANTGIGFATAEQLVKQGQHVILACRNPQKAQEAQNKLRSLDQGQVDVASLDLNSLELTQKAAEEIADKYGSLDVLINNAGLF---SKTKQLTVDGFEQKFGVNYLGHFLLTQKLLPVLKQFPQAHIIHLAS-IAHWVGSIKPNKFRAEGFYNPLFYYGQSKLANLLFSNALAEQLADSSITNNALHPGGVASDIY---------RDLPKPVYAAMKVGLVPTSVPAKLITEMATGDTWQNRNGEYVSAHMPDWKSSHAKNQQLARDLYQQSMNLVEKFL 4Z0T Chain:A ((19-253)) ---LVTGASRGIGYFLSLELAKRGAHVIAVARTVGGLEELDDEIRKLGS-SATLVPLDITDMEALDRLGGTIHERWGKLDILVANAGILGTISPIGHVEAKTFEKVMNINVTSVWRLIRSVDPLLRASDAGRAIMLSSGVAH----------SCRAFWGP---YAASKAAVEVMARCWAEETKKMKLKINSVNPGATRTAMRAQAMPGEDPETLPTPQSVAEKI--VKLADPKLEV----TGKLFDVRQDRFLDYHMP----------------------------

General information: TITO was launched using: RESULT: Template: 4Z0T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1166 -67227 -57.66 -302.82 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -57.66 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.432

(partial model without unconserved sides chains): PDB file : Tito_4Z0T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z0T-query.scw PDB file : Tito_Scwrl_4Z0T.pdb: