Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGLSVMQSLQSLQPRISVAPMMDWTTKDYRFFARLFNPNVVLYTEMVTTGAI----LFGDAKRHLDYNA------------QEHPIVLQLGGSNPQELATCTKMAEDWGYDEVNLNVGCPSDRVQNNKIGACLMAEPDLVAECIHSMQKAVNIPVTVKHRIGIDDMQSYEEMLHFVDTVAATGCTHFVVHARIAILKGLSPKENREVPPLRYEDVYRLKQERPHLTIEINGG----IKTFAETQAHLQHV--DGVMIGREAYHNPYLLAELGQLWNLEAPDRFDIMEQMLPYIEQRLAEGAPLSIITRHILGLFQNLPGARKWRQALSGGNAKTLTDVENAIHNMQAAIIRTEEYLKEHQF 4XP7 Chain:A ((19-279)) --------------KLILAPMVRVGTLPMRLLALDYGADIVYCEELIDLKMIQCKRVVNEVLSTVDFVAPDDRVVFRTCEREQNRVVFQMGTSDAERALAVARLVEN-DVAGIDVNMGCPKQ---------ALLSDPDKIEKILSTLVKGTRRPVTCKIRI----LPSLEDTLSLVKRIERTGIAAIAVHGR--------KREERPQHPVSCE-VIKAIADTLSIPVIANGGSHDHIQQYSDIEDFRQATAASSVMVARAAMWNPSIFLKEG-LRPLE-----EVMQKYIRYAVQ------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4XP7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1275 9521 7.47 41.40 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 7.47 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.383

(partial model without unconserved sides chains): PDB file : Tito_4XP7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XP7-query.scw PDB file : Tito_Scwrl_4XP7.pdb: