Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKMKSPILRLRRLRRTKAIRGLLCENNFSVEDLVQPIFIEESITQPIPISTMPGIYRLPEQDIEQEVKALYALGIRCVMPFGIS--HHKDSMGSDTWSDTGLLVRMIKAIKSSCPEMIVTPDICFCEYTTHGHCGVMIHD-QISNDLTVENLIKQSLAAAKAGADMLAPSGMMDGQVGAIREALDQAGYHNVLIMAHAIKFASALYGPFRVAVD--SNL-VGDRHSYQLDYANTRQALREAALDESEGADLLIVKPGMFYLDILSQLRAQTKLPLAAYQVGGEYAAIKFAAIAKALDERKTVIESITAFKRAGADVIITYFAKDIAIWHNEF 1W5M Chain:A ((11-330)) ------PYTRLRRNRRDDFSRRLVRENVLTVDDLILPVFVLDGVNQRESIPSMPGVERLSIDQLLIEAEEWVALGIPALALFPVTPVEKKSLDAAEAYNPEGIAQRATRALRERFPELGIITDVCLDPFTTHGQCGILDDDGYVLNDVSIDVLVRQALSHAEAGAQVVAPSDMMDGRIGAIREALESAGHTNVRVMAYSAKYASAYYGPFRDAVGSASNLGKGNKATYQMDPANSDEALHEVAADLAEGADMVMVKPGMPYLDIVRRVKDEFRAPTFVYQVSGEY-AMHMGAIQNGWLAESVILESLTAFKRAGADGILTYFAKQAA------

General information: TITO was launched using: RESULT: Template: 1W5M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1711 -35907 -20.99 -114.35 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -20.99 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_1W5M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1W5M-query.scw PDB file : Tito_Scwrl_1W5M.pdb: