Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLVLGGLSAAIMAVSGSVMAADFVAGKDYTVIANPGKVEVP--GKIEVREFFWYGCLHCFKLEPHMQTWLKQIPSDVRFVRTPAAMNKVWEQGARTYYTSEALGVRKRTHLPLFHAIQVNGQ---QIFDQASAAKFFTRYGVPEQKFNSTYNSFAVTAKVAESNKLAQQYQLTGVPAVVVNGKYVVQGEDGKVT-QVLNYLIEKERKAK 2REM Chain:A ((6-190)) -------------------------VVGEDYVEIPD-GRPFAPLAGKIEVVEIFGYTCPHCAHFDSKLQAWGARQAKDVRFTLVPAVFGGVWDPFARAYLAADVLGVAKRSHTAMFEAIHEKGSVPIQNVGPDELAVFYAGYGVQPDRFVATFNGPEVEKRFQAARAYALKVRPVGTPTIVVNGRYMVTGHDFEDTLRITDYLVSRERAAS

General information: TITO was launched using: RESULT: Template: 2REM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 883 -9668 -10.95 -54.01 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -10.95 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.524

(partial model without unconserved sides chains): PDB file : Tito_2REM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2REM-query.scw PDB file : Tito_Scwrl_2REM.pdb: