Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKQQILVDIKDDSMSSNETTTGFKPKKSVALSGQVAGNTALCTVGRSGNDLHYRGYDILDLAAGSEFEEVAHLLVHGKLPNKAELKAYKAKLKALRGLPAALKTALEQLPTSAHPMDVMRTGVSVLGCLTPEHEDHNEAGAKDIADKLMASLGSMLLYWYHFSNNGRRIEVETDDDSIAAHFLHLLHGEKPCEEWIQAMHTSLILYAEHEFNASTFTSRVVAGTGSDMYSAITGGIGALRGPKHGGANEVA---FVIQQRYDNPDEAEADIRKRIENKEVVIGFGHPVYTVSDPRNEVIKKVAHDLAEAQENTKMYL-IAERLEAVMKEV---KNMFPNLDWFSAVSYHLMGVPTAMFTPLFVIARTAGWSAHVIEQRQDG-KIIRPSANYTGPENLEFKPLAERG 1VGM Chain:A ((6-378)) ----------------------------SRGLENVIIKTTGLTYIDGINGILRYRGYDINDLVNYASYEELIHLMLYGELPNRQQLNQIKGIINESFEVPEQVISTIFSMPRNCDAIGMMETAFGILASIY--DPKWNRATNKELAVQIIAKTATITANIYR-AKEGLKPKIPEPSESYAESFLAATFGKKPTQEEIKAMDASLILYTDHEVPASTTAALVASSTLSDMYSCIVAALAALKGPLHGGAAEEAFKQFVEIGSVENADKWFEE--KIIKGKSRLMGFGHRVYKTYDPRAKIFKTLAKSFAEKNENVKKYYEIAERIEKLGVDTFGSKHIYPNTDFYSGIVFYALGFPIYMFTSLFALSRVLGWLAHIIEYVEEQHRLIRPRALYIGPEKREFKPIELR-

General information: TITO was launched using: RESULT: Template: 1VGM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1907 -131539 -68.98 -360.38 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -68.98 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.478

(partial model without unconserved sides chains): PDB file : Tito_1VGM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VGM-query.scw PDB file : Tito_Scwrl_1VGM.pdb: