Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMNKQPQNSALVVVDVQNGFTPGGNLAVADADTIIPTINQLAGCFENVVLTQDWHPDNHISFAANHPGKQPFETIELDYGSQVLWPKHCIQGTHDAEFHPDLNIPTAQLIIRKGFHAHIDSYSAFMEADHTTMTGLTGYLKERGIDTVYVVGIATDFCVAWTALDAVKQGFKTLVIEDACKG-IDLNGSLEQAWQTMQQQGVIRIQSTDLLNEC 3EEF Chain:A ((4-152)) ----------ALVVVDMVNEFI-HGRLATPEAMKTVGPARKVIETFRRSGLPVVYVNDSH------YPDDPEIR----------IWGRHSMKGDDGSEVIDEIRPSAGDYVLEK--HA----YSGFY------GTNLDMILRANGIDTVVLIGLDADICVRHTAADALYRNYRIIVVEDAVAARIDPN---------------------------

General information: TITO was launched using: RESULT: Template: 3EEF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 789 -106468 -134.94 -719.38 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -134.94 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.345

(partial model without unconserved sides chains): PDB file : Tito_3EEF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EEF-query.scw PDB file : Tito_Scwrl_3EEF.pdb: