Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQTETMLDVADNSGARRVQCIKVLGGSHRRYASVGDIIKVTVKEAIPRARVKKGDVMNAVVVRTKFGIRRPDGSVIRFDDNAAVILNNN-KAPIATRIFGPVTRELRIEQFMKIISLAPEVL 5GAG Chain:L ((1-123)) MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIKEAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGPVTRELRSEKFMKIISLAPEVL

General information: TITO was launched using: RESULT: Template: 5GAG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 622 -73043 -117.43 -598.71 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain L : 0.96 3D Compatibility (PKB) : -117.43 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.96 QMean score : 0.575

(partial model without unconserved sides chains): PDB file : Tito_5GAG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GAG-query.scw PDB file : Tito_Scwrl_5GAG.pdb: