Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGQQLVFKSKNALTDLTAIPDFVSYKSRVESDGGVIYDQQAVLDVLQFIFQNSIPETDILSATSAAWGVKFDPVTKVITKLYNLFN-PAGDIIIQNGTMNAIHTTEVDGKPSLYAGGTSTLYGYSSGKFAIANPIAHTIHHVPARSGYGASALLFPLQTLFNKEGFDASTDPAKVSTDYVAVDQRLTRNATTNNDPNTWNEAHRFWGASVGVDGNLNGSRIGYASNALTTGTFIYKDGAQSNSNSTVPNIKPTQQADQRLYLMSNFTVAGVRGNYYLGYIFENWLLNNGTDSIAKALSLRAKTKYR 1NCC Chain:N ((132-231)) ------------------------------------------------------------------------PVTEINTWARNILRTQESECVCHNGVCPVVFT---DG--SATGPAETRIYYFKEGKILKWEPLAGTAKHIEECSCYGERAEI----TCTCRDNWQGSNRPV-IRIDPVAM----------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1NCC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain N - contact count / total energy / energy per contact / energy per residue : 447 -2424 -5.42 -24.48 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain N : 0.59 3D Compatibility (PKB) : -5.42 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.159

(partial model without unconserved sides chains): PDB file : Tito_1NCC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NCC-query.scw PDB file : Tito_Scwrl_1NCC.pdb: