Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFPHHTEHHAIQRSGWLRASVLGANDGIISVTSLIMGMAASGASSHTLFITCVAGLISGATSMAAGEYISVKSQEDIEKSDLAIEAKELKKYPQKELDELTQIYISRGLSKELAKEVAIQLTTHDALGAHARDEIGIHENTAANPIQAALSSAASFSFGAFFPMLAILFSPEHLIMPSVLITGIAALAILGALSSYFAGTSKIKGSLRITLWGILAMAFSSWIGSLFNVTPL 5J73 Chain:A ((6-56)) -----------------------------------------------------------------TSDYIIEQIQRDQEEARKKVEE-------AEERLERVKEASKRGVSSDQLLDLIRELA--------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5J73.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 37 -4420 -119.46 -86.67 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.47 3D Compatibility (PKB) : -119.46 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.47 QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_5J73.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5J73-query.scw PDB file : Tito_Scwrl_5J73.pdb: