Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNKNIGCWLLLAGSSSVCAMQPLDDQSLAAATGQNGLTLGIQADQVKFNQVALIDTNGIASTS--YNSKAGLIIAGNSTNPVPGIEFIKAAVSTNPSFNIAIDTDAGGGNPFLNLAVTMGSDVNGIRLLPFSVYLAPSTSLPSPSDYALTSYAPKSIFSSGTTVNTGVKELIRSTGNLDINFVQTNKPRLNIQLGHAAQSVMVKFGGAIQSICSAASGCPITLVSDNTGATFGFKFAGTNASTGFVLDGFYAGVDPTGLTIGNIGVSSKFDASLNNVTLGNLGTQSTTTFNNLPNGSMGSFGVTGASVTDFKMKVSGF 4GIO Chain:B ((54-84)) -------------------------------------------------YKVDWLDKDGFVLRDVLNEDYQALRIPAGQE-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4GIO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 25 -14 -0.54 -0.47 target 2D structure prediction score : 0.83 Monomeric hydrophicity matching model chain B : 0.47 3D Compatibility (PKB) : -0.54 2D Compatibility (Sec. Struct. Predict.) : 0.83 1D Compatibility (Hydrophobicity) : 0.47 QMean score : 0.091

(partial model without unconserved sides chains): PDB file : Tito_4GIO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GIO-query.scw PDB file : Tito_Scwrl_4GIO.pdb: