Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIEKFSVEKVLSVHRWTPTLFSFTMTRPSHFKFTAGQFARIGLKVGDELIVRAYSVVSSPFDETLEFFSIVVPDGAFTSNLQHLKVGDELYLEKIPYGYLTLARYQQPLP-HDLWLLATGTGLAPFLSMLQDFETWSNYQKINLVYSVRTAAELAYVDRIQEIAETFGEGHLG------FKFIPIITRDPSAPLHERLPVLIENGELEKVAGL-ELSPASSHVMLCGNPQMVDDTKEALKRCGLTMNRR-GEGNIAVENYW 4FK8 Chain:A ((18-266)) ----KFDTATVLSVHHWTDTLFSFTCTRDQALRFNNGEFTMVGLEVDGKPLTRAYSIVSPNYEEHLEFFSIKVQNGPLTSRLQHLKVGDPVLIGKKPTGTLVADNL---LPGKTLWMLSTGTGLAPFMSIIRDPDIYERFDKVVLTHTCRLKGELAYMDYIKH--DLPGHEYLGDVIREKLVYYPTV--------EGRITDLIASGKLFTDLDMPPFSPEQDRVMLCGSTAMLKDTTELLKKAGLVEGKNSAPGHYVIE---

General information: TITO was launched using: RESULT: Template: 4FK8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1226 -77556 -63.26 -332.86 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -63.26 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.466

(partial model without unconserved sides chains): PDB file : Tito_4FK8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FK8-query.scw PDB file : Tito_Scwrl_4FK8.pdb: