Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRKNAIAALLLLPSFSYAATVLSAPPELNNKSYVLMDYETGQILASKNENEKLAPASMTKMMTSYIIEQKLLKGELTENEQVRMNESAWCKGS---SSESCMYVPLNGTATVLEMLRGIIIQSGNDASKAMAEHIAGNEGTFAHMMNQEAKRIGMTNTQFINSTGMPAEGHYSTAKDMAVLAQHIIKDSSKYYPIYSEKEFTFNGIKQGNRNALLYTDP-SVDGLKTGHTDEAGYCLTTSSKRGPMRLISVIFGTPSMSERADQTRALLAWGFANFETANVQPANQVLAKAKVWFGKENEVQVGLAENFNVTMPKGKADGIKTQLVVQPN-LNAPLQKGQVVGKLVASLDGKVIAEKPLVALKPVEEAGFFARLIDHIKQFFSNLF 3MZD Chain:A ((7-357)) --------------------TMIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRMKDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNF----------------

General information: TITO was launched using: RESULT: Template: 3MZD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2033 58586 28.82 169.32 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : 28.82 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_3MZD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MZD-query.scw PDB file : Tito_Scwrl_3MZD.pdb: